The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression

• DOI: 10.1111/tpj.12443
• Publication Date: 2014-01-24T09:25:07Z
• Authors: Alberto Coego; Esther Brizuela; Pablo Castillejo; Sandra Ruíz; Csaba Koncz; Juan C. del Pozo; Manuel Piñeiro; José A. Jarillo; Javier Paz‐Ares; José León
• Keywords: Transcriptional Activation; 0301 basic medicine; DNA, Complementary; Arabidopsis thaliana; Biología; Genetic Vectors; Arabidopsis; Gene Expression; Germination; Plant Roots; 03 medical and health sciences; Plant Growth Regulators; Genes, Reporter; transcription factors; Functional screening; BIOQUIMICA Y BIOLOGIA MOLECULAR; Transcription factors; Transgenes; Promoter Regions, Genetic; Estradiol; Arabidopsis Proteins; Agricultura; transgenic lines collection; Conditional overexpression; Functional genomics; TRANSPLANTA consortium; Plants, Genetically Modified; Transgenic lines collection; functional screening; Phenotype; Seedlings; Seeds; functional genomics; conditional overexpression; Abscisic Acid; Transcription Factors

Transcription factors (TFs) are key regulators of gene expression in all organisms. In eukaryotes, TFs often represented by functionally redundant members large families. Overexpression might prove a means to unveil the biological functions TFs; however, constitutive overexpression frequently causes severe developmental defects, preventing their functional characterization. Conditional strategies help overcome this problem. Here, we report on TRANSPLANTA collection Arabidopsis lines, each expressing one 949 under control β-estradiol-inducible promoter. Thus far, 1636 independent homozygous representing an average 2.6 lines for every TF, have been produced inducible 634 TFs. Along with GUS-GFP reporter, randomly selected were tested and confirmed conditional transgene upon β-estradiol treatment. As proof concept exploitation resource, β-estradiol-induced proliferation root hairs, dark-induced senescence, anthocyanin accumulation dwarfism observed conditionally full-length cDNAs encoding RHD6, WRKY22, MYB123/TT2 MYB26, respectively, agreement previously reported phenotypes conferred these Further screening performed other allowed identification involved different plant processes, illustrating that is powerful resource characterization For instance, ANAC058 TINY/AP2 TF identified as modulators ABA-mediated germination potential, RAP2.10/DEAR4 was regulator cell death hypocotyl-root transition zone. Seeds deposited at Nottingham Stock Centre further distribution.