The Vat locus encodes for a CC‐NBS‐LRR protein that confers resistance to Aphis gossypii infestation and A. gossypii‐mediated virus resistance

• DOI: 10.1111/tpj.12690
• Publication Date: 2014-10-06T07:32:53Z
• Authors: Catherine Dogimont; Veronique Chovelon; Jerome Pauquet; Adnane Boualem; Abdelhafid Bendah...
• Keywords: Aphis gossypii; Cucumis

Aphis gossypii is a polyphagous sucking aphid and vector for many viruses. In Cucumis melo, dominant locus, Vat, confers high level of resistance to infestation viruses transmitted by this vector. To investigate the mechanism underlying double resistance, we first genetically dissected Vat locus. We delimited single gene that encodes coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein type. validate genetic data, transgenic lines expressing were generated assessed resistance. analysis, Vat-transgenic plants resistant A. as well gossypii-mediated virus transmission. When infected mechanically, infection occurred on both non-transgenic control plants. These results confirmed cloned CC-NBS-LRR mediates using This also invokes separate recognition response phases in which phase involves interaction an elicitor molecule from plant. The not specific blocks infection. Sequence analysis alleles suggests major role unusual conserved LRR gossypii.