The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt‐stress response in Arabidopsis thaliana

• DOI: 10.1111/tpj.12797
• Publication Date: 2015-02-19T15:13:56Z
• Authors: Miaomiao Tian; Lijuan Lou; Lijing Liu; Feifei Yu; Qingzhen Zhao; Huawei Zhang; Yaorong Wu; Sanyuan Tang; Ran Xia; Baoge Zhu; Giovanna Serino; Qi Xie
• Keywords: Brefeldin A

Salt stress is a detrimental factor for plant growth and development. The response to salt has been shown involve components in the intracellular trafficking system, as well of ubiquitin-proteasome system (UPS). In this article, we have identified Arabidopsis thaliana little reported ubiquitin ligase involved salt-stress response, which named STRF1 (Salt Tolerance RING Finger 1). member RING-H2 finger proteins demonstrate that it activity vitro. We also show localizes mainly at plasma membrane endosomes. strf1-1 loss-of-function mutant seedlings exhibit accelerated endocytosis roots, altered expression several genes system. Moreover, protein inhibitor, brefeldin A (BFA), treatment increased BFA bodies mutant. This showed tolerance salt, ionic osmotic stresses, reduced accumulation reactive oxygen species during stress, AtRbohD, encodes nicotinamide adenine dinucleotide phosphate (NADPH) oxidase H2 O2 production. conclude trafficking-related ligase, helps respond by monitoring (ROS)