Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations

• DOI: 10.1111/tpj.12878
• Publication Date: 2015-05-20T10:07:55Z
• Authors: Diarmuid S. Ó'Mao...; Bennett Thomson; Andrea Raganelli; Samuel E. Wuest; Patrick T. Ryan; Kamila Kwaśniewska; Cristel C. Carles; Emmanuelle Graciet; Frank Wellmer
• Keywords: Gene regulatory network

Summary Understanding how flowers develop from undifferentiated stem cells has occupied developmental biologists for decades. Key to unraveling this process is a detailed knowledge of the global regulatory hierarchies that control transitions, cell differentiation and organ growth. These may be deduced gene perturbation experiments, which determine effects on expression after specific disruption gene. Here, we tested experimental strategies experiments during Arabidopsis thaliana flower development. We used artificial mi RNA s (ami s) disrupt functions key floral regulators, expressed them under various inducible promoter systems are widely in plant research community. To able perform genome‐wide with stage‐specific resolution using also generated series induction allow collection hundreds synchronized buds single plant. Based our results, propose performing dynamic flowers, outline they combined versions system dissect network underlying