E3 ubiquitin ligase gene CMPG1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)

• DOI: 10.1111/tpj.12966
• Publication Date: 2015-08-19T16:08:45Z
• Authors: Yanfei Zhu; Yingbo Li; Fei Fei; Zongkuan Wang; Wei Wang; Aizhong Cao; Yuan Liu; Shuang Han; Liping Xing; Haiyan Wang; Wei Chen; Sanyuan Tang; Xiahe Huang; Qianhua Shen; Qi Xie; Xiue Wang
• Keywords: Common wheat

Summary Powdery mildew is one of the most devastating wheat fungal diseases. A diploid relative, Haynaldia villosa L., highly resistant to powdery mildew, and its genetic resource resistances, such as Pm21 locus, now widely used in breeding. Here we report cloning a resistance gene from H. , designated CMPG 1–V that encodes U–box E3 ubiquitin ligase. Expression was induced leaf stem upon inoculation with Blumeria graminis f. sp. tritici ( Bgt ) fungus, presence essential for rapid induction expression. has conserved key residues ligase, possesses ligase activity vitro vivo . localized nucleus, endoplasmic reticulum, plasma membrane partially trans ‐Golgi network/early endosome vesicles. Transgenic over‐expressing showed improved broad‐spectrum at seedling adult stages, associated an increase expression salicylic acid‐responsive genes, H 2 O accumulation, cell‐wall protein cross‐linking infection sites, increased when treated acid, abscisic acid These results indicate involvement defense responses fungus wheat, particularly disease resistance, suggest association reactive oxidative species phytohormone pathway 1 – V ‐mediated resistance.