AAE13 encodes a dual‐localized malonyl‐CoA synthetase that is crucial for mitochondrial fatty acid biosynthesis

• DOI: 10.1111/tpj.13130
• Publication Date: 2016-02-02T16:43:35Z
• Authors: Xin Guan; Basil J. Nikolau
• Keywords: Fatty acid synthesis; Mitochondrial matrix; Acetyl-CoA Carboxylase; Acyl carrier protein; Malonyl-CoA

Malonyl-CoA is a key intermediate in number of metabolic processes associated with its role as substrate acylation and condensation reactions. These types reactions occur plastids, the cytosol mitochondria, although carboxylation acetyl-CoA known mechanism for generating distinct plastidial cytosolic pools, origin mitochondrial malonyl-CoA pool still unclear. In this study we demonstrate that synthetase encoded by Arabidopsis AAE13 (AT3G16170) gene localized both mitochondria. isoforms are translated from two transcripts, one contains does not contain mitochondrial-targeting pre-sequence. Whereas protein essential, due to presence redundant system provided carboxylase, essential plant growth. Phenotypes aae13-1 mutant transgenically reversed only if pre-sequence present ectopically expressed proteins. The exhibits typical phenotypes deficiency fatty acid synthase system, namely depleted lipoylation H subunit photorespiratory enzyme glycine decarboxylase, increased accumulation glycolate reduced levels sucrose. Most these alterations, morphological changes, when grown non-photorespiratory condition (i.e. 1% CO2 atmosphere), demonstrating they consequence photorespiration inability generate lipoic mitochondrially synthesized acids.