Membrane microdomains and the cytoskeleton constrain AtHIR1 dynamics and facilitate the formation of an AtHIR1‐associated immune complex

• DOI: 10.1111/tpj.13480
• Publication Date: 2017-01-13T03:22:33Z
• Authors: Xueqin Lv; Yanping Jing; Jianwei Xiao; Yongdeng Zhang; Yingfang Zhu; Russell Julian; Jinxing Lin
• Keywords: Lipid microdomain

Arabidopsis hypersensitive-induced reaction (AtHIR) proteins function in plant innate immunity. However, the underlying mechanisms by which AtHIRs participate immunity remain elusive. Here, using VA-TIRFM and FLIM-FRET, we revealed that AtHIR1 is present membrane microdomains co-localizes with microdomain marker REM1.3. Single-particle tracking analysis cytoskeleton, especially microtubules, restrict lateral mobility of at plasma facilitate its oligomerization. Furthermore, protein proximity index measurements, fluorescence cross-correlation spectroscopy, biochemical experiments demonstrated formation complex upon pathogen perception requires intact cytoskeleton. Taken together, these findings suggest cytoskeleton constrain dynamics, promote oligomerization, increase efficiency interactions components response to pathogens, thus providing valuable insight into defense-related responses plants.