OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice

• DOI: 10.1111/tpj.13595
• Publication Date: 2017-05-10T07:02:48Z
• Authors: Natsuko I. Kobayashi; Naoki Yamaji; Hiroki Yamamoto; Kaoru Okubo; Hiroki Ueno; Alex Costa; Keitaro Tanoi; Hideo Matsumura; Miho Fujii‐Kashino; Tomoki Horiuchi; Mohammad Al Nayef; Sergey Shabala; Gynheung An; Jian Feng Ma; Tomoaki Horie
• Keywords: 2. Zero hunger; 0303 health sciences; Symporters; Protoplasts; Sodium; Oryza; [object Object]; Salt Tolerance; Phloem; Sodium Chloride; Plant Roots; Plant Leaves; Mutagenesis, Insertional; 03 medical and health sciences; Stress, Physiological; Xylem; HKT; Oryza sativa; Na+ exclusion; phloem; salt tolerance; xylem; Cation Transport Proteins; [object Object; Plant Proteins

Summary Salt tolerance quantitative trait loci analysis of rice has revealed that the SKC 1 locus, which is involved in a higher K + /Na ratio shoots, corresponds to Os HKT 1;5 gene encoding Na ‐selective transporter. However, physiological roles exposed salt stress remain elusive, and no disruption mutants have been characterized date. In this study, we dissected two independent T‐ DNA insertional mutants. Measurements ion contents tissues 22 tracer imaging experiments showed loss‐of‐function salt‐stressed roots triggers massive accumulation shoots. stress‐induced increases transcript were observed basal stems, including nodes. Immuno‐staining using an anti‐Os peptide antibody indicated localized cells adjacent xylem roots. Additionally, direct introduction leaf sheaths also demonstrated involvement unloading sheaths. Furthermore, was be present plasma membrane found localize phloem diffuse vascular bundles Together with characteristic allocation blade developing immature mutants, these results suggest novel function mediating exclusion prevent transfer young blades. Our findings further demonstrate crucial over growth stages rice, protection next generation seeds as well vital blades under stress.