Arabidopsis lipid droplet‐associated protein (LDAP) – interacting protein (LDIP) influences lipid droplet size and neutral lipid homeostasis in both leaves and seeds

• DOI: 10.1111/tpj.13754
• Publication Date: 2017-10-30T09:10:48Z
• Authors: Michal Pyc; Yingqi Cai; Satinder K. Gidda; Olga Yurchenko; Sunjung Park; Franziska K. Kret...; Till Ischebeck; Oliver Valerius; Gerhard H. Braus; Kent D. Chapman; John M. Dyer; Robert T. Mullen
• Keywords: Lipid droplet; Oleosin

Cytoplasmic lipid droplets (LDs) are found in all types of plant cells; they derived from the endoplasmic reticulum and function as a repository for neutral lipids, well serving remodelling signalling. However, mechanisms underlying formation, steady-state maintenance turnover LDs, particularly non-seed tissues, relatively unknown. Previously, we showed that LD-associated proteins (LDAPs) family plant-specific, LD surface-associated coat required proper biogenesis LDs homeostasis vegetative tissues. Here, screened yeast two-hybrid library using Arabidopsis LDAP3 isoform 'bait' an effort to identify other novel protein constituents. One candidate LDAP3-interacting was At5g16550, which is plant-specific unknown termed LDIP (LDAP-interacting protein). Using combination biochemical cellular approaches, show targets specifically surface, contains discrete amphipathic α-helical targeting sequence, participates both homotypic heterotypic associations with itself LDAP3, respectively. Analysis T-DNA knockdown knockout mutants decrease abundance increase variability size leaves, concomitant increases total content. Similar phenotypes were observed seeds, enlarged amounts seed oil. Collectively, these data new player biology modulates leaves seeds.