A cytological approach to studying meiotic recombination and chromosome dynamics in Arabidopsis thaliana male meiocytes in three dimensions

• DOI: 10.1111/tpj.13942
• Publication Date: 2018-04-22T10:24:35Z
• Authors: Aurélie Hurel; Dylan Phillips; Nathalie Vrielynck; Christine Mézard; Mathilde Grelon; Nicolas Christoph...
• Keywords: Meiocyte; Synapsis; Synaptonemal complex

Summary During meiotic prophase I chromosomes undergo dramatic conformational changes that accompany chromosome condensation, pairing and recombination between homologs. These include the anchoring of telomeres to nuclear envelope their clustering form a bouquet. In plants, these events have been studied illustrated in intact meiocytes species with large genomes. Arabidopsis thaliana is an excellent genetic model which major molecular pathways control synapsis homologs uncovered. Yet study dynamics hampered by current cytological methods disrupt three‐dimensional (3D) architecture nucleus. Here we set up protocol preserve 3D configuration A. meiocytes. We showed this technique compatible use variety antibodies label structural proteins were able highlight presence clustered initiation centers at periphery. By using fluorescence situ hybridization also behavior during pre‐meiotic G 2 I, revealing existence telomere bouquet male meiosis. addition number its volume vary greatly, thus complexity I. Finally, probes subtelomeric regions individual chromosomes, revealed differential localization behaviors ends. Our opens new areas research for investigating