A novel tomato F‐box protein, SlEBF3, is involved in tuning ethylene signaling during plant development and climacteric fruit ripening

• DOI: 10.1111/tpj.13976
• Publication Date: 2018-05-24T06:19:55Z
• Authors: Heng Deng; Julien Pirrello; Yao Chen; Nan Li; Sihua Zhu; Ximena Chirinos; Mondher Bouzayen; Yongsheng Liu; Mingchun Liu
• Keywords: Climacteric

Summary Ethylene is instrumental to climacteric fruit ripening and EIN 3 BINDING F‐ BOX ( EBF ) proteins have been assigned a central role in mediating ethylene responses by regulating 3/ EIL degradation Arabidopsis. However, the mode of action tomato s ethylene‐dependent processes like remains unclear. Two novel genes, Sl 4 , were identified genome, displayed ripening‐associated expression pattern suggesting its potential involvement controlling response during ripening. downregulated lines failed show obvious ripening‐related phenotypes likely due functional redundancy among family members. By contrast, SlEBF3 overexpression exhibited pleiotropic ethylene‐related alterations, including inhibition ripening, attenuated triple‐response delayed petal abscission. Yeast‐two‐hybrid system bimolecular fluorescence complementation approaches indicated that interacts with all known and, consistently, total protein levels decreased fruits, supporting idea reduced sensitivity defects are 3‐mediated proteins. Moreover, regulated 1 via feedback loop, which supposes tuning signaling responses. Overall, study reveals gene thus providing new target for modulating fleshy