Metabolite profiling and genome‐wide association studies reveal response mechanisms of phosphorus deficiency in maize seedling

• DOI: 10.1111/tpj.14160
• Publication Date: 2018-11-25T10:29:48Z
• Authors: Bowen Luo; Peng Ma; Zhi Nie; Xiao Zhang; Xuan He; Xin Ding; Xing Feng; Quanxiao Lu; Zhiyong Ren; Haijian Lin; Yuanqi Wu; Yaou Shen; Suzhi Zhang; Ling Wu; Dan Liu; Guangtang Pan; Tingzhao Rong; Shibin Gao
• Keywords: Metabolite profiling; Profiling (computer programming); Genome-wide Association Study; Genetic Association

SUMMARY Inorganic phosphorus (Pi) is an essential element in numerous metabolic reactions and signaling pathways, but the molecular details of these pathways remain largely unknown. In this study, metabolite profiles maize ( Zea mays L.) leaves roots were compared between six low‐Pi‐sensitive lines low‐Pi‐tolerant under Pi‐sufficient Pi‐deficient conditions to identify genes associated with low‐Pi stress response. Results showed that Pi deprivation concentrations nucleic acids, organic acids sugars increased, phosphorylated metabolites, certain amino lipid metabolites nitrogenous compounds decreased. The levels secondary involved plant immune reactions, including benzoxazinoids flavonoids, significantly different plants grown conditions. Among them, 11 most stable significant differences low‐ normal‐Pi based on coefficient variation CV ). Isoleucine alanine for identification Pi‐sensitive Pi‐resistant inbred lines. With correlation morphological traits five low‐Pi‐responding consensus simultaneously mined by combining genome‐wide association study GWAS induced deficiency seedlings also validated reverse‐transcription quantitative polymerase chain reaction RT ‐ qPCR Moreover, further a recombinant line RIL ) population, which glucose‐6‐phosphate‐1‐epimerase encoding gene mediated yield correlated availability. Together, our results provide framework understanding processes underlying responses give multiple insights into improving efficiency use maize.