A wheat dominant dwarfing line with Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line

• DOI: 10.1111/tpj.14168
• Publication Date: 2018-11-22T18:01:50Z
• Authors: Linhe Sun; Wenlong Yang; Yafei Li; Qiangqiang Shan; Xiaobin Ye; Dongzhi Wang; Kang Yu; Weiwen Lu; Peiyong Xin; Zhong Pei; Xiaoli Guo; Dongcheng Liu; Jiazhu Sun; Kehui Zhan; Jinfang Chu; Aimin Zhang
• Keywords: Dwarfing; Bulked segregant analysis; Gibberellic acid

Dwarfing and semi-dwarfing are important agronomic traits that have great potential for the improvement of wheat yields. Rht12, a dominant gibberellic acid (GA)-responsive dwarfing gene from gamma-ray-induced mutant Karcagi 522M7K, is located in long arm chromosome 5A, which closely linked with locus Xwmc410. Rht12 likely an ideal GA biosynthesis deactivation research common wheat. However, information on sequence lacking. In this study, significantly shortened stem cell length decreased biosynthetic components. Using bulked segregant RNA-Seq, 660k single nucleotide polymorphism chip detection, newly developed simple repeat markers, was mapped to 11.21-Mb region at terminal end 5AL, found be Xw5ac207SSR marker 10.73-Mb fragment deletion all homologous plants. Transcriptome analyses remaining 483-kb showed higher expression TraesCS5A01G543100 encoding metabolic enzyme 2-β-dioxygenase plants than high stalk plants, suggesting reduces plant height by activating TaGA2ox-A14. Taken together, our findings will promote cloning functional studies