A wheat dominant dwarfing line with Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line
Dwarfing
Bulked segregant analysis
Gibberellic acid
DOI:
10.1111/tpj.14168
Publication Date:
2018-11-22T18:01:50Z
AUTHORS (16)
ABSTRACT
Dwarfing and semi-dwarfing are important agronomic traits that have great potential for the improvement of wheat yields. Rht12, a dominant gibberellic acid (GA)-responsive dwarfing gene from gamma-ray-induced mutant Karcagi 522M7K, is located in long arm chromosome 5A, which closely linked with locus Xwmc410. Rht12 likely an ideal GA biosynthesis deactivation research common wheat. However, information on sequence lacking. In this study, significantly shortened stem cell length decreased biosynthetic components. Using bulked segregant RNA-Seq, 660k single nucleotide polymorphism chip detection, newly developed simple repeat markers, was mapped to 11.21-Mb region at terminal end 5AL, found be Xw5ac207SSR marker 10.73-Mb fragment deletion all homologous plants. Transcriptome analyses remaining 483-kb showed higher expression TraesCS5A01G543100 encoding metabolic enzyme 2-β-dioxygenase plants than high stalk plants, suggesting reduces plant height by activating TaGA2ox-A14. Taken together, our findings will promote cloning functional studies
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (56)
CITATIONS (74)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....