Dual‐color oligo‐FISH can reveal chromosomal variations and evolution in Oryza species

• DOI: 10.1111/tpj.14522
• Publication Date: 2019-09-08T08:54:31Z
• Authors: Xiaoyu Liu; Shang Sun; Ying Wu; Yong Zhou; Siwei Gu; Hengxiu Yu; Chuandeng Yi; Minghong Gu; Jiming Jiang; Bao Liu; Tao Zhang; Zhiyun Gong
• Keywords: Oryza; Hybridization probe; DNA Barcoding

Fluorescence in situ hybridization using probes based on oligonucleotides (oligo-FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo-FISH that allow the of each 12 pairs chromosomes rice (Oryza sativa). These comprised 25 717 (green) 215 (red) oligos (45 nucleotides), respectively, generated 26 distinct FISH signals can be used as barcode to uniquely label chromosomes. Standard karyotypes were established this system both mitotic meiotic Moreover, dual-color was characterize diverse chromosomal abnormalities. Oligo-FISH analyses these various wild Oryza species revealed from AA, BB or CC genomes specific intense similar those rice, while with EE genome less FF gave no signal. Together, will powerful studying variations evolution genus Oryza.