Arabidopsis REM16 acts as a B3 domain transcription factor to promote flowering time via directly binding to the promoters of SOC1 and FT

• DOI: 10.1111/tpj.14807
• Publication Date: 2020-05-11T10:14:55Z
• Authors: Yanchong Yu; Longfei Qiao; Jiacai Chen; Yongheng Rong; Yuhang Zhao; Xiankui Cui; Jinpeng Xu; Xiaomin Hou; Chun‐Hai Dong
• Keywords: 0303 health sciences; 03 medical and health sciences; Arabidopsis Proteins; Arabidopsis; MADS Domain Proteins; Flowers; Transcription Factors, General; Plants, Genetically Modified; Promoter Regions, Genetic; Transcriptome; Transcription Factors

Actin depolymerizing factor (ADF) is a key modulator for dynamic organization of actin cytoskeleton. Interestingly, it was found that the ADF1 gene silencing delays flowering, but its mechanism remains unclear. In this study, used as bait to screen interacting proteins by yeast two-hybrid (Y2H) system. One them, REM16 transcription identified. As one AP2/B3-like transcriptional family members, contains two B3 domains and transcript levels kept increasing during floral transition stage. Overexpression accelerates flowering while flowering. Gene expression analysis indicated activation genes such CONSTANS (CO), FLOWERING LOCUS T (FT), LEAFY (LFY) SUPPRESSOR OF OVEREXPRESSION (SOC1) were upregulated in overexpression lines, suppression C (FLC) decreased. contrast, lines contained lower CO, FT, LFY SOC1 higher FLC compared with wild-type plants. It proved could directly bind promoter regions FT vitro vivo assays. Genetic supported acts upstream pathways. All these studies provided strong evidence demonstrating promotes activating FT.