Precision transcriptomics of viral foci reveals the spatial regulation of immune‐signaling genes and identifies RBOHD as an important player in the incompatible interaction between potato virus Y and potato

• DOI: 10.1111/tpj.14953
• Publication Date: 2020-08-09T20:47:15Z
• Authors: Tjaša Lukan; Maruša Pompe‐Novak; Špela Baebler; Magda Tušek‐Žnidarič; Aleš Kladnik; Maja Križnik; Andrej Blejec; Maja Zagorščak; Katja Stare; Barbara Dušak; Anna Coll; Stephan Pollmann; Karolina Morgiewicz; Jacek Hennig; Kristina Gruden
• Keywords: Hypersensitive response

SUMMARY Whereas the activation of resistance (R) proteins has been intensively studied, downstream signaling mechanisms leading to restriction pathogen remain mostly unknown. We studied immunity network response conditioned by potato Ny‐1 gene against virus Y. analyzed processes in cell death zone and surrounding tissue on biochemical expression levels order reveal spatiotemporal regulation immune response. show that transcriptional is dependent salicylic acid (SA). For some genes completely lost SA‐deficient line, whereas other a different response, indicating multiple connections between hormonal modules. The induction NADPH oxidase RBOHD occurs specifically lesion border during In plants with silenced , functionality perturbed spread not arrested at site infection. required for spatial accumulation SA, conversely under SA. Using spatially resolved RNA‐seq, we also identified an UDP‐glucosyltransferase, another component feedback SA biosynthesis, thus deciphering novel aspect signaling.