Hydroperoxide lyase modulates defense response and confers lesion‐mimic leaf phenotype in soybean (Glycine max (L.) Merr.)

• DOI: 10.1111/tpj.15002
• Publication Date: 2020-09-30T08:30:28Z
• Authors: Yaqi Wang; Meifeng Liu; Dongdong Ge; Javaid Akhter Bhat; Yawei Li; Jiejie Kong; Kang Liu; Tuanjie Zhao
• Keywords: Wild type

Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) are two important members of P450 enzymes metabolizing hydroperoxy fatty acid to produce jasmonates aldehydes respectively, which function in response diverse environmental developmental stimuli. However, their exact roles soybean have not been clarified. In present study, we identified a lesion-mimic mutant named NT302, exhibits etiolated phenotype together with chlorotic spontaneous lesions on leaves at R3 podding stage. The underlying gene was as GmHPL encoding by map-based cloning strategy. Sequence analysis demonstrated that single nucleotide mutation created premature termination codon (Gln20-Ter), resulted truncated protein NT302. RNA significantly reduced NT302 mutant, while genes AOS branch the 13-LOX pathway were up-regulated exhibited higher susceptibility bacterial leaf pustule (BLP) disease, but increased resistance against common cutworm (CCW) pest. induced MeJA, wounding, CCW wild type soybean. Virus silencing (VIGS) GhHPL gave rise similar phenotypes upland cotton, coupled upregulation expression JA biosynthesis JA-induced genes. Our study provides evidence competition exist between HPL branches oxylipin metabolism soybean, thereby plays essential modulation plant development defense.