Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus

• DOI: 10.1111/tpj.15003
• Publication Date: 2020-10-13T19:08:14Z
• Authors: Shan Tang; Dong‐Xu Liu; Shaoping Lu; Liangqian Yu; Yuqing Li; Shengli Lin; Long Li; Zhuolin Du; Xiao Liu; Xiao Li; Wei Ma; Qing‐Yong Yang; Liang Guo
• Keywords: Ethyl methanesulfonate; Erucic acid

Brassica napus is an important oilseed crop in the world, and mechanism of seed oil biosynthesis B. remains unclear. In order to study generate germplasms for breeding, ethyl methanesulfonate (EMS) mutant population with ~100 000 M2 lines was generated using Zhongshuang 11 as parent line. The EMS-induced genome-wide mutations M2-M4 plants were assessed. average number including single nucleotide polymorphisms insertion/deletion 21 177, 28 675 17 915, respectively. effects on gene function predicted mutants, We screened seeds from 98 113 lines, 9415 content fatty acid mutants identified. further confirmed 686 altered advanced generation (M4 seeds). Five representative M4 increased oleic re-sequenced, potential causal variations FAD2 ROD1 genes This a large scale EMS population, identified could provide useful genetic resources improvement composition future.