Fine mapping and cloning of the major seed protein quantitative trait loci on soybean chromosome 20

• DOI: 10.1111/tpj.15658
• Publication Date: 2022-01-03T09:19:08Z
• Authors: Christina E. Fliege; Russell A. Ward; Pamela Vogel; Hanh Nguyen; Truyen Quach; Ming Guo; João Paulo Gomes ...; Lucas Borges dos ...; James E. Specht; Tom E. Clemente; Matthew E. Hudson; Brian W. Diers
• Keywords: Cloning (programming); Trait

Soybean [Glycine max (L.) Merr.] is a unique crop species because it has high levels of both protein and oil in its seed. Of the many quantitative trait loci (QTL) controlling soybean seed content, alleles cqSeed protein-003 QTL on chromosome 20 exert greatest additive effect. The high-protein allele exists cultivated wild (Glycine soja Siebold & Zucc.) germplasm. Our objective was to fine map this enable positional-based cloning underlying causative gene(s). Fine mapping achieved by developing testing series populations which chromosomal region surrounding segregating high- versus low-protein gradually narrowed, using marker-based detection recombinant events. resultant 77.8 kb interval directly sequenced from G. source compared with reference genome identify structural sequence polymorphisms. An insertion/deletion variant detected Glyma.20G85100 found have near-perfect +/− concordance high/low-protein genotypes inferred for parents published populations. indel structure concordant an evolutionarily recent insertion TIR transposon into gene lineage. Seed significantly greater expressing RNAi hairpin downregulation element two independent events relative control null segregant lineages. We conclude that within CCT domain encoded accounts high/low QTL.