AhNPR3 regulates the expression of WRKY and PR genes, and mediates the immune response of the peanut (Arachis hypogaea L.)

• DOI: 10.1111/tpj.15700
• Publication Date: 2022-02-06T05:58:18Z
• Authors: Suoyi Han; Ximeng Zhou; Lei Shi; Huayang Zhang; Yun Geng; Yuanjin Fang; Han Xia; Hua Liu; Pengcheng Li; Shuzhen Zhao; Lijuan Miao; Lei Hou; Zhongxin Zhang; Jing Xu; Changle Ma; Zhenyu Wang; Hongyan Li; Zheng Zheng; Bingyan Huang; Wenzhao Dong; Jun Zhang; Fengshou Tang; Shaojian Li; Meng Gao; Xinyou Zhang; Chuanzhi Zhao; Xingjun Wang
• Keywords: WRKY protein domain; Systemic Acquired Resistance; Pathogenesis-related protein

Systemic acquired resistance is an essential immune response that triggers a broad-spectrum disease throughout the plant. In present study, we identified peanut lesion mimic mutant m14 derived from ethyl methane sulfonate-mutagenized pool of cultivar "Yuanza9102." Brown lesions were observed in leaves seedling stage to maturity. Using MutMap together with bulked segregation RNA analysis approaches, G-to-A point mutation was exon region candidate gene Arahy.R60CUW, which homolog AtNPR3 (Nonexpresser PR genes) Arabidopsis. This caused transition Gly Arg within C-terminal transactivation domain AhNPR3A. The AhNPR3A showed no effect induction genes when treated salicylic acid. Instead, resulted upregulation WRKY and several genes, including pathogenesis-related thaumatin- chitinase-encoding consistent resistant phenotype leaf spot disease. Further study on will provide valuable insights into understanding molecular mechanism systemic peanut. Moreover, our results indicated combination effective method for identifying mutants.