KNOX II transcription factor HOS59 functions in regulating rice grain size

• DOI: 10.1111/tpj.15709
• Publication Date: 2022-02-15T17:24:57Z
• Authors: Minghao Sheng; Xuelian Ma; Jiyao Wang; Tianxi Xue; Zhongqiu Li; Yaxin Cao; Xinyue Yu; Xinyi Zhang; Yonghong Wang; Wenying Xu; Zhen Su
• Keywords: Chromatin immunoprecipitation

Plant Knotted1-like homeobox (KNOX) genes encode homeodomain-containing transcription factors. In rice (Oryza sativa L.), little is known about the downstream target of KNOX Class II subfamily proteins. Here we generated chromatin immunoprecipitation (ChIP)-sequencing datasets for HOS59, a member subfamily, and characterized genome-wide binding sites HOS59. We conducted trait ontology (TO) analysis 9705 identified genes, found that multiple TO terms are related to plant structure morphology stress traits. ChIP-quantitative PCR (qPCR) was validate some key genes. Meanwhile, our IP-MS showed HOS59 closely associated with BELL family proteins, grain size regulators (OsSPL13, OsSPL16, OsSPL18, SLG, etc.), epigenetic modification factors such as OsAGO4α OsAGO4β, proteins involved in small interfering RNA-mediated gene silencing. Furthermore, employed CRISPR/Cas9 editing transgenic approaches generate hos59 mutants overexpression lines, respectively. Compared wild-type plants, have longer grains increased glume cell length, loose architecture, drooping leaves, while lines smaller size, erect lower height. The qRT-PCR results mutation led upregulation size-related OsSPL13, PGL2. summary, indicate may be repressor negatively regulating etc. possible interaction improve understanding regulatory networks.