GhBZR3 suppresses cotton fiber elongation by inhibiting very‐long‐chain fatty acid biosynthesis

• DOI: 10.1111/tpj.15852
• Publication Date: 2022-06-02T16:37:37Z
• Authors: Zemin Shi; Xia Chen; Huidan Xue; Tingting Jia; Funing Meng; Yunfei Liu; Xiaomin Luo; Guanghui Xiao; Shengwei Zhu
• Keywords: Brassinosteroid; Gossypium; Elongation

The BRASSINAZOLE-RESISTANT (BZR) transcription factor is a core component of brassinosteroid (BR) signaling and involved in the development many plant species. BR essential for initiation elongation cotton fibers. However, mechanism BR-regulating fiber function BZR poorly understood Gossypium hirsutum L. (cotton). Here, we identified family protein referred to as GhBZR3 cotton. Overexpression Arabidopsis caused shorter root hair length, hypocotyl cell indicating that negatively regulates elongation. Pathway enrichment analysis from VIGS-GhBZR3 plants found fatty acid metabolism degradation might be regulatory pathway primarily controlled by GhBZR3. Silencing expression resulted taller height well longer very-long-chain (VLCFA) content was also significantly increased silenced compared with wild type. GhKCS13 promoter, key gene VLCFA biosynthesis, contains two binding sites. results yeast one-hybrid, electrophoretic mobility shift, luciferase assays revealed directly interacted promoter suppress expression. Taken together, these indicate reducing biosynthesis. This study not only deepens our understanding development, but highlights potential improving length growth using its related genes future breeding programs.