CLE3 and its homologs share overlapping functions in the modulation of lateral root formation through CLV1 and BAM1 in Arabidopsis thaliana

• DOI: 10.1111/tpj.16103
• Publication Date: 2023-01-11T07:35:01Z
• Authors: Satoru Nakagami; Tsuyoshi Aoyama; Yoshikatsu Sato; Taiki Kajiwara; Takashi Ishida; Shinichiro Sawa
• Keywords: Lateral root; Primordium

Lateral roots are important for a wide range of processes, including uptake water and nutrients. The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) 1 ~ 7 peptide family their cognate receptor CLV1 have been shown to negatively regulate lateral root formation under low-nitrate conditions. However, little is known about how CLE signaling regulates formation. A persistent obstacle in research functional redundancies, which makes analyses difficult. To address this problem, we generate the cle1 septuple mutant (cle1 7-cr1, cr stands allele generated with CRISPR/Cas9). 7-cr1 exhibits longer normal Specifically, density increased, primordia initiation found be accelerated. Further analysis shows that cle3 single slightly roots. On other hand, plants overexpress CLE2 CLE3 exhibit decreased lengths. explore receptor(s) CLE3, analyze lengths clv1 barely any meristem 1(bam1) double mutant. Mutating both BAM1 causes roots, but not each In addition, genetic reveals epistatic CLE3. Furthermore, gene expression LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKE (LBD/ASL) genes, promote formation, upregulated bam1. We therefore propose peptides perceived by mediate through LBDs regulation.