Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation

• DOI: 10.1111/tpj.16227
• Publication Date: 2023-04-03T07:50:09Z
• Authors: Junzhi Wang; Lei Lian; Jiale Qi; Yuhang Fang; Alex Nyporko; Qin Yu; Lianyang Bai; Lang Pan
• Keywords: Acetolactate synthase; Transcription

Frequent herbicide use selects for resistance in weeds. Cytochrome P450s are important detoxification enzymes responsible plants. We identified and characterized a candidate P450 gene (BsCYP81Q32) from the problematic weed Beckmannia syzigachne to test whether it conferred metabolic acetolactate synthase-inhibiting herbicides mesosulfuron-methyl, bispyribac-sodium, pyriminobac-methyl. Transgenic rice overexpressing BsCYP81Q32 was resistant three herbicides. Equally, ortholog OsCYP81Q32 more mesosulfuron-methyl. Conversely, an knockout generated using CRISPR/Cas9 enhanced mesosulfuron-methyl sensitivity rice. Overexpression of resulted metabolism transgenic seedlings via O-demethylation. The major metabolite, demethylated chemically synthesized displayed reduced herbicidal effect Moreover, transcription factor (BsTGAL6) shown bind key region promoter activation. Inhibition BsTGAL6 expression by salicylic acid treatment B. plants consequently changed whole plant response Sequence polymorphisms may explain higher versus susceptible Collectively, present study reveals evolution herbicide-metabolizing resistance-endowing its regulation economically weedy species.