Single‐cell RNA sequencing analysis of the embryogenic callus clarifies the spatiotemporal developmental trajectories of the early somatic embryo in Dimocarpus longan

• DOI: 10.1111/tpj.16319
• Publication Date: 2023-05-26T18:09:20Z
• Authors: Shuting Zhang; Chen Zhu; Xueying Zhang; Mengyu Liu; Xiaodong Xue; Chunwang Lai; Xu Xuhan; Yukun Chen; Zihao Zhang; Zhongxiong Lai; Yuling Lin
• Keywords: Plant Somatic Embryogenesis Techniques; Sapindaceae; Sequence Analysis, RNA; Gene Expression Regulation, Plant; Gene Expression Profiling; Embryonic Development; Transcriptome; Plant Proteins

Plant embryogenic calli (ECs) can undergo somatic embryogenesis to regenerate plants. This process is mediated by regulatory factors, such as transcription factors and specifically expressed genes, but the precise molecular mechanisms underlying at single-cell level remain unclear. In this study, we performed high-resolution RNA sequencing analysis determine cellular changes in EC of woody plant species Dimocarpus longan (longan) clarify continuous cell differentiation trajectories transcriptome level. The highly heterogeneous cells were divided into 12 putative clusters (e.g., proliferating, meristematic, vascular, epidermal clusters). We determined cluster-enriched expression marker genes found that overexpression gene GDSL ESTERASE/LIPASE-1 inhibited hydrolysis triacylglycerol. addition, stability autophagy was critical for longan. pseudo-timeline elucidated from early embryonic division vascular during Moreover, key transcriptional regulators associated with fates revealed. ETHYLENE RESPONSIVE FACTOR 6 characterized a heat-sensitive factor negatively regulates under high-temperature stress conditions. results study provide new spatiotemporal insights resolution.