Genome‐wide association study reveals the genetic basis for petal‐size formation in rapeseed (Brassica napus) and CRISPR‐Cas9‐mediated mutagenesis of BnFHY3 for petal‐size reduction

• DOI: 10.1111/tpj.16609
• Publication Date: 2023-12-30T15:24:11Z
• Authors: Ruisen Wang; Yafei Li; Shiqi Xu; Qi Huang; Mengxin Tu; Yang Zhu; Haiyan Cen; Jie Dong; Lixi Jiang; Xiangtan Yao
• Keywords: Genome size

Petals in rapeseed (Brassica napus) serve multiple functions, including protection of reproductive organs, nutrient acquisition, and attraction pollinators. However, they also cluster densely at the top, forming a thick layer that absorbs reflects considerable amount photosynthetically active radiation. Breeding genotypes with large, small, or even petal-less varieties, requires knowledge primary genes for allelic selection manipulation. our current understanding petal-size regulation is limited, lack markers pre-breeding materials hinders targeted breeding. Here, we conducted genome-wide association study on petal size using 295 diverse accessions. We identified 20 significant single nucleotide polymorphisms 236 associated variation. Through cross-analysis genomic transcriptomic data, focused 14 specific genes, from which molecular diverging features can be developed. Leveraging CRISPR-Cas9 technology, successfully generated quadruple mutant Far-Red Elongated Hypocotyl 3 (q-bnfhy3), exhibited smaller petals compared to wild type. Our provides insights into genetic basis offers abundant potential The q-bnfhy3 unveiled novel role FHY3 orthologues regulating addition previously reported functions.