AAO2 impairment enhances aldehyde detoxification by AAO3 in Arabidopsis leaves exposed to UV‐C or Rose‐Bengal

• DOI: 10.1111/tpj.16985
• Publication Date: 2024-08-27T18:50:41Z
• Authors: Zhadyrassyn Nurbe...; Sudhakar Srivastava; Zai Du Nja; Kusum Khatri; Jaykumar Patel; Babita Choudhary; Veronika Turečková; Miroslav Strnad; Edyta Zdunek‐Zast...; Rustem Omarov; Dominic Standing; Moshe Sagi
• Keywords: Rose bengal; Detoxification

Among the three active aldehyde oxidases in Arabidopsis thaliana leaves (AAO1-3), AAO3, which catalyzes oxidation of abscisic-aldehyde to abscisic-acid, was shown recently function as a reactive detoxifier. Notably, aao2KO mutants exhibited less senescence symptoms and lower accumulation, such acrolein, benzaldehyde, 4-hydroxyl-2-nonenal (HNE) than wild-type exposed UV-C or Rose-Bengal. The effect AAO2 expression absence on detoxification by AAO3 and/or AAO1 studied comparing response plants single-functioning aao1 mutant (aao1S), mutants, aao3 (aao3Ss). aao3Ss similar accumulation chlorophyll content treated with In contrast, aao1S higher that resulted remaining leaves, indicating enhanced activity mutants. support this notion, employing specific substrate marker for revealed compared abscisic-acid level accumulated unstressed stressed genotypes indicates is cause better stress resistance Employing sulfuration process (known activate oxidases) wild-type, aao2KO, molybdenum-cofactor sulfurase (aba3-1) WT employs processes essential level, resulting aao2KO.