Global nuclear reorganization during heterochromatin replication in the giant‐genome plant Nigella damascena L.

• DOI: 10.1111/tpj.17063
• Publication Date: 2024-10-21T18:09:36Z
• Authors: Eugene A. Arifulin; Dmitry V. Sorokin; Nadezhda A. Anoshina; Maria A. Kuznetsova; Anna A. Valyaeva; Daria M. Potashni...; Denis O. Omelchenko; Veit Schubert; Tatyana D. Kolesn...; Eugene V. Sheval
• Keywords: Interphase; Replication timing

SUMMARY Among flowering plants, genome size varies remarkably, by >2200‐fold, and this variation depends on the loss gain of noncoding DNA sequences that form distinct heterochromatin complexes during interphase. In plants with giant genomes, most chromatin remains condensed interphase, forming a dense network threads called interphase chromonemata. Using super‐resolution light electron microscopy, we studied ultrastructure chromonemata after replication in root meristem nuclei Nigella damascena L. During S‐phase, undergoes transient decondensation locally at sites. Due to abundance heterochromatin, leads robust disassembly chromonema meshwork general reorganization nuclear morphology visible even conventional microscopy. After replication, recondenses, restoring structure. Thus, show giant‐genome induces global reorganization.