LTD1 plays a key role in rice tillering regulation through cooperation with CycH1;1 and TFB2 subunits of the TFIIH complex

Bimolecular fluorescence complementation
DOI: 10.1111/tpj.70119 Publication Date: 2025-04-01T13:42:50Z
ABSTRACT
SUMMARY Tillering contributes greatly to grain yield in rice ( Oryza sativa ). At present, many genes involved tillering regulation have been cloned and characterized. However, the identification of more novel is still necessary fully understand molecular mechanisms regulating tillering. In this study, we isolated a low ‐ dwarf 1 ltd1 ) mutant indica rice. Map‐based cloning MutMap analysis showed that candidate gene LTD1 LOC_Os01g19760 encodes putative FAM91A1 protein with an unknown function plants. ‐complementation ‐RNAi confirmed responsible for phenotype . The localized plasma membrane, endoplasmic reticulum, multi‐vesicular bodies. Furthermore, interaction colocalization assays interacts both TFB2 subunit core subcomplex CycH1;1 cyclin‐dependent kinase‐activating kinase (CAK) TFIIH complex, also CycH1;1. qRT‐PCR demonstrated expression levels most related cell cycle are changed significantly tiller buds, flow cytometry revealed there polyploid nuclei leaves roots, suggesting could be regulation. Taken together, our findings indicated plays key role by involvement through cooperation subunits TFIIH. This work sheds light on biological important agronomic traits
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