In vitro comparison of cryopreserved and liquid platelets: potential clinical implications

• DOI: 10.1111/trf.12915
• Publication Date: 2014-11-05T07:02:41Z
• Authors: Lacey Johnson; Michael C. Reade; Ryan A. Hyland; Shereen Tan; Denese C. Marks
• Keywords: Microparticle

Background Platelet ( PLT ) concentrates can be cryopreserved in dimethyl sulfoxide DMSO and stored at −80° C for 2 years. These storage conditions improve availability both rural military environments. Previous phenotypic vitro studies of PLTs are limited by comparison to fresh liquid‐stored , rather than over their clinically relevant shelf life. Further, nothing is known the effect reconstituting plasma a variety temperatures. Study Design Methods Apheresis were either room temperature 5 days or with 5% . Cryopreserved thawed 37° reconstituted (stored different temperatures) compared expired In assays performed assess glycoprotein expression, activity, microparticle content, function. Results Compared liquid storage, had reduced expression key receptors GPIbα GPIIb However, proportion expressing activation markers CD62P CD 63 was similar between expiry. components contained significantly higher numbers phosphatidylserine‐ tissue factor–positive microparticles these time clot formation increased thrombin generation. Conclusion There distinct differences also have an enhanced hemostatic activity. Knowledge will essential understanding outcomes clinical trial comparing various durations.