Red blood cells for transfusion in patients with sepsis: respective roles of unit age and exposure to recipient plasma

• DOI: 10.1111/trf.14170
• Publication Date: 2017-06-01T03:32:45Z
• Authors: Philippe Chadebech; Gwellaouen Bodivit; Keyvan Razazi; Christophe de Vas...; Laurence Pellé; Nicolas Burin‐des...; Thibault Bocquet; Philippe Bierling; Rachid Djoudi; Armand Mekontso‐D...; France Pirenne
• Keywords: Senescence; Haptoglobin; Blood plasma

BACKGROUND Red blood cell (RBC) storage in banks is not exempt from cellular injury. Alterations observed on RBCs freshly isolated units can rapidly appear circulation. The transfusion of old units, even if this a controversial issue, could therefore have adverse effects the recipient. We wanted to determine respective duration and recipient plasma for into patients with severe sepsis. STUDY DESIGN AND METHODS Eleven stored RBC were sampled at various time points, approximately Days 3 8 (referred as fresh RBCs) 38 42 (old tested coincubation experiments obtained 13 sepsis 17 healthy donors controls. after 24 or 48 hours 37°C detection senescence markers (phosphatidylserine exposure, calcium influx, reactive oxygen species decrease size) without exposure plasma. RESULTS confirmed that 42‐day refrigerated alone (without any incubation plasma) had no significant effect marker detected. By contrast, ex vivo samples altered both RBCs, much larger regardless used (sepsis vs. control). CONCLUSION show main factor affecting age rather than clinical status