Sequence analyses of variable cytomegalovirus genes for distinction between breast milk– and transfusion‐transmitted infections in very‐low‐birth‐weight infants

• DOI: 10.1111/trf.14920
• Publication Date: 2018-10-19T10:34:34Z
• Authors: Yasumi Furui; Naoji Yamagishi; Ichiro Morioka; Rikizo Taira; Kosuke Nishida; Shohei Ohyama; Hisayuki Matsumoto; Yuji Nakamachi; Takashi Hasegawa; Keiji Matsubayashi; Tadashi Nagai; Masahiro Satake
• Keywords: Cytomegalovirus; Betaherpesvirinae

BACKGROUND Cytomegalovirus (CMV) transmission to very‐low‐birth‐weight infants (VLBWIs) sometimes induces serious clinical symptoms. Although breast milk is considered a major source of transmission, transfusion‐transmitted CMV (TT‐CMV) infection often suspected when disease develops after transfusion. Thus, it clinically important distinguish between and milk–transmitted infections. STUDY DESIGN AND METHODS Study A: The incidence acquired was prospectively investigated in 65 VLBWIs. B: To determine the routes 18 TT‐CMV–suspected VLBWIs who had been reported our hemovigilance system, we performed polymerase chain reaction for DNA fed and/or repository blood samples related transfused leukoreduced products. Furthermore, evaluated identity strains patients' urine/blood by sequence analyses variable genes UL139 UL146 . RESULTS Acquired found 4 (6.2%). detected 12 14 cases, which available. sequence‐matching rates both were 100% or nearly 11 patients. In contrast, patients negative. CONCLUSION principally transmitted through risk TT‐CMV seems be extremely low using Sequence are useful evaluating routes.