Platelet‐derived extracellular vesicles convey mitochondrial DAMPs in platelet concentrates and their levels are associated with adverse reactions

• DOI: 10.1111/trf.15300
• Publication Date: 2019-04-12T04:05:34Z
• Authors: Genevieve Marcoux; Audrey Magron; Caroline Sut; Audree Laroche; Sandrine Laradi; Hind Hamzeh‐Cognasse; Isabelle Allaeys; Ophelie Cabon; Anne‐Sophie Julien; Olivier Garraud; Fabrice Cognasse; Eric Boilard
• Keywords: Extracellular vesicles; Extracellular Vesicles; Neutrophil Extracellular Traps; Damp; Mitochondrial ROS

BACKGROUND Whereas platelet transfusion is a common medical procedure, inflammation still occurs in fraction of transfused individuals despite the absence any apparent infectious agents. Platelets can shed membrane vesicles, called extracellular vesicles (EVs), some which contain mitochondria (mito+EV). With its content damage‐associated molecular pattern (DAMP), mitochondrion stimulate innate immune system. Mitochondrial DNA (mtDNA) recognized DAMP detected milieu numerous inflammatory conditions and concentrates. We hypothesized that platelet‐derived encapsulated EVs may represent reservoir mtDNA. STUDY DESIGN AND METHODS Herein, we explored implication mito+EVs occurrence mtDNA quantified concentrate supernatants induced or did not induce adverse reactions. RESULTS observed were abundant concentrates, more concentrates A significant correlation (r s = 0.73; p < 0.0001) between mito+EV levels concentrations was found. However, there nonsignificant without −0.11; 0.5112). The majority into EVs. CONCLUSION This study suggests EVs, such as those convey mitochondrial DAMPs, be useful biomarker for prediction potential risk Moreover, our work implies investigations are necessary to determine whether causal pathogenic role opposed solution.