Pooling, room temperature, and extended storage time increase the release of adult‐specific biologic response modifiers in platelet concentrates: a hidden transfusion risk for neonates?

• DOI: 10.1111/trf.15827
• Publication Date: 2020-04-27T20:03:13Z
• Authors: Miriam Waubert de...; Lina K. Sciesielski; Oliver Meyer; Zhi‐Jian Liu; Chiara‐Aiyleen Badur; Helge Schönfeld; Rudolf Tauber; Axel Pruß; Martha C. Sola‐Vi...; Christof Dame
• Keywords: Platelet Transfusion; Thrombogenicity

BACKGROUND Adult donor platelets (PLTs) are frequently transfused to prevent or stop bleeding in neonates with thrombocytopenia. There is evidence for PLT transfusion–related morbidity and mortality, leading the hypothesis on immunomodulatory effects of transfusing adult PLTs into neonates. Candidate factors biologic response modifiers (BRMs) that expressed at higher rates than neonatal PLTs. This study investigated whether storage conditions preparation methods impact release those differentially BRMs. STUDY DESIGN AND METHODS Pooled concentrates (PCs) apheresis PCs (APCs) were stored under agitation up 7 days room temperature (RT) 2 8°C. The BRMs CCL5/RANTES, TGFβ1, TSP1, DKK1 measured PCsʼ supernatant, lysate, corresponding plasma. function was assessed by light transmission aggregometry. RESULTS Concerning method, concentrations found pooled compared APCs. In supernatants, CCL5, significantly increased, both over standard (≤4 days) extended times (7 days). Each four showed an twofold increase concentration after RT cold (CS). no difference aggregation capacity. CONCLUSION analysis shows adult‐specific during lowest short‐ CS Our points strategies reducing exposure sick can be specifically associated morbidity.