Refrigeration of apheresis platelets in platelet additive solution (PAS‐E) supports in vitro platelet quality to maximize the shelf‐life

• DOI: 10.1111/trf.16489
• Publication Date: 2021-07-16T13:13:28Z
• Authors: Lacey Johnson; Shuchna Vekariya; Ben Wood; Shereen Tan; Christopher Roan; Denese C. Marks
• Keywords: Blood product; Platelet Transfusion; Plateletpheresis

Abstract Background Refrigeration, or cold‐storage, of platelets may be beneficial to extend the limited shelf‐life conventionally stored and support transfusion protocols in rural military areas. The aim this study was compare morphologic, metabolic, functional aspects apheresis at room‐temperature (RT) cold conditions, either plasma supplemented with platelet additive solution (PAS). Study design methods Double‐dose were collected 100% 40% plasma/60% PAS‐E using Trima platform. One component from each group RT (20–24°C) refrigerated (2–6°C). Platelets tested over a 21‐day period. Results concentration decreased by approximately 30% all groups during 21 days storage ( p > .05). Cold‐storage reduced glycolytic metabolism, pH maintained above minimum specification (>6.4) for only when PAS. surface phenotype composition supernatant differentially affected temperature solution. Functional responses (aggregation, agonist‐induced receptor activation, clotting time) improved influence residual assay dependent. Conclusion In vitro quality is time, temperature, Cold‐storage, particularly PAS, better maintains key phenotypic, parameters prolonged storage.