Assessment of the soluble proteins HMGB1, CD40L and CD62P during various platelet preparation processes and the storage of platelet concentrates: The BEST collaborative study

• DOI: 10.1111/trf.17200
• Publication Date: 2022-12-01T15:26:35Z
• Authors: Fabrice Cognasse; Hind Hamzeh Cognasse; Marie Ange Eyraud; Amélie Prier; Charles Antoine A...; Pierre Tiberghien; Stephane Begue; Dirk de Korte; Eric Gouwerok; Andreas Greinacher; Konstanze Aurich; Femke Noorman; Larry Dumont; Kathleen Kelly; Marc Cloutier; Renée Bazin; Rebecca Cardigan; Sian Huish; Peter Smethurst; Dana Devine; Peter Schubert; Lacey Johnson; Denese C. Marks
• Keywords: Blood preservation

Abstract Background Structural and biochemical changes in stored platelets are influenced by collection processing methods. This international study investigates the effects of platelet (PLT) storage conditions on HMGB1, sCD40L, sCD62P protein levels concentrate supernatants (PCs). Study Design/Methods PC ( n = 3748) were collected each centre using identical centrifugation methods 9) tested centrally ELISA/Luminex platform. Apheresis versus buffy coat (BC‐PC) method, plasma PAS RT cold (4°C) investigated. We focused preparation collecting samples during early (RT: day 1–3; cold: 1–5) late 4–7; 7–10) time points. Results concentrations similar periods, regardless solution (BC‐PC BC‐PC PAS‐E) or temperature. During without PAS, sCD40L CD62P increased significantly (+33% +41%, respectively) depending temperature (22 vs. 4°C). However, PAS‐E, decreased (−31% −20%, respectively), Contrastingly, method appeared to have greater impact HMGB1 release duration. These data highlight increases these parameters differences between temperatures. Conclusions The mechanism/intracellular pathways appear differ from sCD40L. extent which affect patient outcomes, particularly post‐transfusion increment adverse events, warrants further investigation clinical trials with various therapeutic indications.