OA3‐AM23‐TU‐12 | Six Novel RHCE Alleles Identified in Investigation of Donor Antigen Typing Discrepancies

• DOI: 10.1111/trf.50_17554
• Publication Date: 2023-10-12T10:32:37Z
• Authors: L. Rahorst; S. Vege; J. Fuertes; A. Burgos; R. Velliquette; C. Thompson; J. Dugger; C. Westhoff; G. Ochoa‐Garay
• Keywords: Sanger sequencing

New alleles are often discovered when targeted genotyping predicts antigen positive and serology shows negative, weak, or variable reactivity. Donor discrepancy resolution is important for correct labeling. We investigated five donor samples with RhCE between PreciseType HEA. Serologic testing was done by standard tube methods using Immucor Gamma-clone, Ortho BioClone, Quotient ALBAclone Bio-Rad Seraclone antisera. DNA isolated from white blood cells. RHCE BeadChip (Immucor) Sanger sequencing of exons 1-10 flanking intron regions were performed. RHCE*E-specific (S1, S3) long-range PCR RHCE*C followed allele-specific (S5) to phase variant sequences. Table 1 summarizes the donors findings. S1–S4 predicted C–E+c+e+ HEA discrepancies in serologic E (S1–S3) e (S4) antigens. S1 had reactivity Anti-E reagents (Ortho nonreactive, weakly reactive, strongly reactive). confirmed results, identified c.667G >T change (p.Val223Phe) exon 5, placed it on ce (ceMO). However, E-specific found c.667T cE. S2 showed very nonreactive). no c.885G >A (p.Met295Ile) 6. S3 RBCs typed E– Anti-E. variant, while revealed c.341G (p.Arg114Gln) 3. c.341A S4 e– Anti-e. No detected BeadChip. Sequencing c.1115T (p.Leu372Stop) 8. S5 C+E–c+e+ HEA, but C− Anti-C reacted Anti-C. variant. c.19C (p.Arg7Trp) c.512A >C (His171Pro) Exon 4. Further c.19T c.512C Ce. report six novel samples: cE(667T), cE(885A), cE(341A), ce(1115A), Ce(512C), ce(19T). The cE(667T) similar that reported ce(667T), Ce(667T). E−/E+vw phenotype associated cE(885A) consistent weak partial D Del phenotypes RHD*885A. has been expression low prevalence JAL antigen. Unfortunately, we unable test JAL. RHCE*ce(1115A) presumed null given premature stop codon e− typing. Similar c encoded ce(512G), Ce(512C) encodes C. While RHD results D, doesn’t appear affect ce, evidenced strong c+