Proliferation of psychrotrophic bacteria in cold‐stored platelet concentrates

• DOI: 10.1111/vox.13640
• Publication Date: 2024-04-18T01:29:11Z
• Authors: Sandra Ramirez‐Arcos; Dilini Kumaran; Andrew Cap; Kristin Michelle ...; Marc Cloutier; Justin Ferdin; Ute Gravemann; Patrick Ketter; Patricia Landry; Thea Lu; Truscha Niekerk; Joel Parker; Claudia Renke; Axel Seltsam; Bianca Stafford; Susanne Süssner; Tanja Vollmer; Susann Zilkenat; Carl McDonald
• Keywords: Pseudomonas fluorescens; Colony-forming unit; Psychrotrophic bacteria; Bacterial growth

Abstract Background and Objectives Platelet concentrates (PC) are stored at 20–24°C to maintain platelet functionality, which may promote growth of contaminant bacteria. Alternatively, cold storage PC limits bacterial growth; however, data related proliferation psychotrophic species in cold‐stored (CSP) scarce, is addressed this study. Materials Methods Eight laboratories participated study with a pool/split approach. Two split units were spiked ~25 colony forming (CFU)/PC Staphylococcus aureus , Klebsiella pneumoniae Serratia liquefaciens Pseudomonas fluorescens Listeria monocytogenes . One unit was under agitation 20–24°C/7 days while the second 1–6°C/no for 21 days. sampled periodically determine loads. Five repeated inoculated lyophilized inocula (~30 CFU/mL) S. K. Results All proliferated 20–24°C, reaching concentrations ≤10 9 CFU/mL by day 7. Psychrotrophic P. CSP ~10 6 5 on 10 17 storage, respectively, followed L. reached 2 21. did not grow CSP. Conclusion bacteria, relatively rare contaminants PC, CSP, clinically significant levels (≥10 before 14 storage. Cold reduces risk comparable RBC units. Safety could be further improved implementing detection systems or pathogen reduction technologies if beyond