Serum and Synovial Fluid Serum Amyloid A Response in Equine Models of Synovitis and Septic Arthritis

• DOI: 10.1111/vsu.12531
• Publication Date: 2016-09-01T10:52:07Z
• Authors: Elsa K. Ludwig; R. Brandon Wiese; Megan R. Graham; Amelia J. Tyler; Julie M. Settlage; Stephen R. Werre; Christina S. Pete...; Isis Kanevsky‐Mul...; Linda A. Dahlgren
• Keywords: Immunoassay; Male; Arthritis, Infectious; Serum Amyloid A Protein; Synovitis; 04 agricultural and veterinary sciences; 3. Good health; 0403 veterinary science; Disease Models, Animal; Nephelometry and Turbidimetry; Synovial Fluid; Animals; Female; Horse Diseases; Horses

To investigate the serum and synovial fluid amyloid A (SAA) response in equine models of synovitis septic arthritis to compare handheld validated immunoturbidometric assays for SAA quantification.Controlled, experimental study.Healthy adult horses (n = 9).Synovitis 4) 5) were induced using lipopolysaccharide Staphylococcus aureus, respectively, serial samples collected. Serial cytology was performed both from model submitted bacterial culture. Serum quantified by test assay. Cytologic data compared within between (mixed ANOVA) results category-by-category analysis (weighted kappa coefficient).Synovial total nucleated cell counts protein increased significantly following induction models. remained normal horses. more rapidly than SAA. Agreement 98% when concentrations low (<50 μg/mL) but diverged greater ∼100 μg/mL. Overall, there good agreement 0.824).Serum may be useful adjuncts diagnosing examination a larger sample size is needed before direct numeric comparisons can made.