Efficiency assessment of irrigation as an alternative method for improving the regenerative potential of non‐healing wounds

• DOI: 10.1111/wrr.13013
• Publication Date: 2022-04-05T14:24:43Z
• Authors: Anna Figiel‐Dabro...; Natalia E. Krzesniak; Bartłomiej H. Nos...; Krystyna Domanska...; Anna Sarnowska
• Keywords: Regenerative Medicine

Abstract The application of mesenchymal stem/stromal cells (MSC) in regenerative medicine offers hope for the effective treatment incurable or difficult‐to‐heal diseases. However, it requires development unified protocols both safe and efficient cell acquisition clinical usage. therapeutic effect fat grafts (containing stem cells) non‐healing wounds has been discussed previous studies, although local general anaesthesia. MSC derived from adipose tissue (ASC) could be a less invasive method, delivery lead to more favourable outcomes, which should encourage clinicians use such approaches frequently. Therefore, aim this study was optimise methods ASC isolation, culture administration while maintaining their high survival, proliferation colonisation potential. were isolated by an enzymatic method characterised according International Society Cellular Therapy Federation Adipose Therapeutics Science guidelines. To assess opportunity obtain sufficient number transplantation, long‐term cultures two oxygen concentrations (5% vs. 21%) conducted. For these cultures, population doubling time, cumulative time doublings rate senescence estimated. In developed pre‐defined protocol, can efficiently cultured at physiological (5%), leads faster slower senescence. Subsequently, select optimal minimally direct with irrigator skin dressings analysed. Our results confirmed that presented allow into without losing vitality. Cells propagated described conditions applied non‐invasive (with irrigation system dressings) treat chronic potential alternative supplement approaches.