Early graft failure of GalTKO pig organs in baboons is reduced by expression of a human complement pathway‐regulatory protein

• DOI: 10.1111/xen.12176
• Publication Date: 2015-07-14T21:20:30Z
• Authors: Agnes M. Azimzadeh; Sean S. Kelishadi; Mohamed B. Ezzelarab; Avneesh K. Singh; Tiffany Stoddard; Hayato Iwase; Tianshu Zhang; Lars Burdorf; Evelyn Sievert; Chris Avon; Xiangfei Cheng; David Ayares; Keith A. Horvath; Philip C. Corcoran; Muhammad M. Mohiu...; Rolf N. Barth; David K. C. Cooper; Richard N. Pierson
• Keywords: Immunosuppression; Cumulative incidence

Abstract We describe the incidence of early graft failure ( EGF , defined as loss function from any cause within 3 days after transplant) in a large cohort Gal TKO pig organs transplanted into baboons three centers, and effect additional expression human complement pathway‐regulatory protein, CD 46 or 55 (Gal . hCPRP ). Baboon recipients life‐supporting kidney (n = 7) heterotopic heart 14) grafts received either no immunosuppression 4), one several partial full immunosuppressive regimens 17). Fourteen 5) 9) similar treatment regimens. Immunologic, pathologic, coagulation parameters were measured at frequent intervals. occurred 9/21 (43%). reduced to 7% (1/14; P < 0.01 vs. alone). At 30 mins, deposits more intense which developed (P 0.005). The intensity peri‐transplant platelet activation (as β‐thromboglobulin release) correlated with did cumulative score 0.01). conclude that (i) transgenic on vascular endothelium reduces deposition, (ii) deposition correlate organ failure, (iii) but does not prevent systemic activation. Additional strategies will be required control