Immunogenicity of hepatic differentiated human umbilical cord mesenchymal stem cells promoted by porcine decellularized liver scaffolds

• DOI: 10.1111/xen.12287
• Publication Date: 2017-01-19T13:21:32Z
• Authors: Yi Li; Qiong Wu; Yujia Wang; Li Li; Fei Chen; Yujun Shi; Hong Bu; Ji Bao
• Keywords: Xenotransplantation; Decellularization

Cell-based approaches, including hepatocyte transplantation and tissue-engineered livers, offer promising alternatives are expected to help support patients with liver diseases until or recovery via regeneration of the damaged liver. However, success cell therapies remains dependent on how well cells accepted after is directly related their degree immunogenicity. In this study, hepatic differentiation human umbilical cord mesenchymal stem (hUC-MSCs) was induced in traditional monolayer (2D) culture newly established three-dimensional (3D) aggregation porcine decellularized scaffold (DLS) system (3D-DLS). We investigated immunogenicity these hepatocyte-like vitro. found that differentiated hUC-MSCs expressed higher levels leukocyte antigen-DR (HLA-DR) (P<.05) lost ability inhibit lymphocyte proliferation (P<.05), association a lower level prostaglandin E2 (PGE2 ) interferon-γ (IFN-γ) secretion, compared undifferentiated hUC-MSCs. The 3D-DLS did not show an elevation MHC-II (P>.05), cause obvious lymphocytes proliferation, demonstrated more PGE2 less IFN-γ secretion. Hepatocyte-like presented immunogenic phenotype than 2D also performed immunosuppressive capacity culture.