Contribution of rat embryonic stem cells to xenogeneic chimeras in blastocyst or 8‐cell embryo injection and aggregation

• DOI: 10.1111/xen.12468
• Publication Date: 2018-10-30T13:22:32Z
• Authors: Hiroki Okumura; Anna Nakanishi; Satoshi Toyama; Mai Yamanoue; Kana Yamada; Akane Ukai; Tadahiro Hashita; Takahiro Iwao; Tomomi Miyamoto; Yoh‐ichi Tagawa; Masumi Hirabayashi; Ichiro Miyoshi; Tamihide Matsunaga
• Keywords: Chimera (genetics)

Abstract The ultimate goal of regenerative medicine is the transplantation a target organ generated by patient's own cells. Recently, method generation using pluripotent stem cells ( PSC s) and blastocyst complementation was reported. This approach based on chimeric animal an early embryo s, contribution s to key method's success. However, rate in organs different methods remains unknown. In this study, we used 8‐cell aggregation, injection, injection generate interspecies mice rat embryonic ES ) then investigated differences mouse highest followed order aggregation. neonates difference statistically significant liver. Live functionality confirmed analyzing expression hepatocyte‐derived drug‐metabolizing enzyme. Collectively, these findings indicate that most suitable for ‐derived via generation, particularly