Chemical cross‐linking of xenopericardial biomeshes: A bottom‐up study of structural and functional correlations

• DOI: 10.1111/xen.12506
• Publication Date: 2019-02-28T09:02:33Z
• Authors: Ekaterina A. Greb...; Leonid P. Istranov; Elena V. Istranova; Semyon N. Churbanov; Boris S. Shavkuta; Ruslan I. Dmitriev; Nadezhda N. Verya...; Svetlana L. Kotova; Alexander V. Kurkov; Anatoly B. Shekhter; Peter S. Timashev
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Cross-Linking Reagents; Tissue Engineering; Materials Testing; Transplantation, Heterologous; Animals; Cattle; Iridoids; Pericardium

Abstract Decellularized bovine pericardium (DBP)‐based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after transplantation, various chemical cross‐linking strategies employed. However, structural and functional properties of differ dependence on cross‐linker used. Here, we performed a bottom‐up study alterations DBP‐based following with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide (EDC) genipin. The vitro cytotoxicity tests supported clinical applicability. Their differences (eg roughness, fibre thickness, pore morphology) were evaluated using two‐photon confocal laser scanning, atomic force, scanning electron polarized light microscopies. HMDC EDC samples appeared be roughest. Complex mechanical trials indicated tendency reduced Young’s Modulus anisotropy values DBP upon cross‐linking. lowest was found genipin sample groups. collagenase susceptibility highest for EGDE samples. comparative analysis results allowed us recognize strengths weaknesses each relation particular application.