Scleral defect repair using decellularized porcine sclera in a rabbit model

• DOI: 10.1111/xen.12633
• Publication Date: 2020-07-29T22:34:26Z
• Authors: Fuyan Wang; Long Zhao; Hua Li; Dongfang Li; Mingming Zhou; Qingjun Zhou; Lixin Xie
• Keywords: Decellularization; Sclera; Biocompatibility

Abstract Background The sclera is one of the most commonly used repair materials in ophthalmic plastic surgery and often for supporting, wrapping, filling, pressing during surgery. Although plays an irreplaceable role ophthalmology applications, there are many restrictive factors, such as high costs limited sources. Here, we report use a decellularized porcine (DPS) scleral reconstruction rabbit models. Methods DPS generated by hybrid decellularization protocol was characterized respect histological observation, DNA, α‐gal, GAG, collagen content. mechanical properties were evaluated uniaxial tensile testing. LIVE/DEAD Cell Counting Kit (CCK)‐8 assays performed to assess its vitro cytocompatibility cytotoxicity. In vivo biocompatibility biointegration repairing defect measured slit‐lamp analyses. Immunohistochemical (IHC) staining detect expression CD4, CD8, CD45, CD68, vimentin. Results Through decellularization, major xenoantigen DNA α‐gal efficiently removed while abundant matrix components well preserved DPS. Extracts samples had no inhibitory effects on proliferation HFSFs. Moreover, sign that immune reaction occurred or around transplanted grafts within 28 days animal implantation. Conclusion strategy developed feasible effective. prepared holds great potential injury.