Assessing the exercise‐related kinetics of circulating cell‐free DNA, circulating tumour DNA, DNase I activity and cytokines in patients with solid tumours: A pilot study

• DOI: 10.1113/ep092167
• Publication Date: 2025-03-29T14:06:59Z
• Authors: Elmo W. I. Neuberger; Alexandra Brahmer; Tobias Ehlert; Suzan Botzenhardt; Alfonso De Falco; Birgit Enders; Patricia S. Hähnel; Achim Heintz; Carl C. Schimanski; Thomas Kindler; Perikles Simon
• Keywords: Cell-free fetal DNA

Circulating cell-free DNA (cfDNA), circulating tumour (ctDNA) and inflammatory cytokines have prognostic predictive value in oncology. However, the effects of acute exercise on cfDNA levels are unknown. Here, we explore kinetics cfDNA, ctDNA upon an incremental test a pilot cohort cancer patients compared with healthy control subjects. Patients solid tumours (n = 12) age-matched subjects 6) were recruited to perform all-out cardiopulmonary bicycle test. Blood samples collected before (Pre), directly after (Post) 90 min (+90 min), (Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations), DNase I activity cytokine measured. Cardiopulmonary testing was easily feasible patients, data from eight five available for exploratory statistical evaluation. The increased Pre Post decreased baseline at +90 all concentrations clearly correlated but not group. Neutrophil-associated myeloperoxidase (MPO), calprotectin (MRP 8/14), lipocalin A (NGAL) showed strong responses exercise. percentage ctDNA, detected only one patient, In our study, could safely different entities subsequently run biomarker analyses. Our results hint exercise-triggered release neutrophil-derived patients.