Regulation of pacemaker frequency in the murine gastric antrum

• DOI: 10.1113/jphysiol.2001.012765
• Publication Date: 2002-07-27T12:45:57Z
• Authors: Tae Wan Kim; Elizabeth A. H. B...; Rhonda Hanna; Sang Don Koh; Tamás Ördög; Sean M. Ward; Kenton M. Sanders
• Keywords: Male; Mice, Inbred BALB C; Periodicity; 0303 health sciences; Cultured; Prostaglandin E; 571; Cells; Colforsin; 8-Bromo Cyclic Adenosine Monophosphate; Dinoprostone; Electrophysiology; Mice; 03 medical and health sciences; Receptors; Pyloric Antrum; Animals; Receptors, Prostaglandin E; Female; Inbred BALB C; Cells, Cultured

PGE(2) has been linked to the production of gastric arrhythmias such as tachygastria. The interstitial cells Cajal (ICC) generate electrical rhythmicity in gastrointestinal muscles, and may therefore be a target for muscles. We cultured ICC from murine antrum, verified that were Kit immunoreactive, measured spontaneous slow waves. These events caused by inward (pacemaker) currents not blocked nifedipine. Forskolin 8-bromoadenosine 3':5'-cyclic monophosphate (8-Br-cAMP) reduced frequency pacemaker waves intact antral effects forskolin 8-Br-cAMP inhibitors protein kinase A, suggesting cAMP direct on activity. mimicked ICC, but increased slow-wave Therefore, chronotropic specific prostaglandin EP receptor agonists examined. Butaprost ONO-AE1-329, EP(2) EP(4) agonists, Sulprostone (EP(3)>EP(1) agonist), GR63799, ONO-AE-248 (EP(3) agonists) enhanced frequencies sulprostone SC-19220, an EP(1) antagonist. observations suggest positive muscles are mediated EP(3) stimulation. dependent upon relative expression receptors and/or proximity sources PGE(2).