Influence of pre‐exercise muscle glycogen content on exercise‐induced transcriptional regulation of metabolic genes

• DOI: 10.1113/jphysiol.2002.016832
• Publication Date: 2002-07-27T12:45:57Z
• Authors: Henriette Pilegaard; Charlotte Keller; Adam Steensberg; Jørn Wulff Helge; Bente Klarlund Pe...; Bengt Saltin; P. Darrell Neufer
• Keywords: PDK4; UCP3; Vastus lateralis muscle

Transcription of metabolic genes is transiently induced during recovery from exercise in skeletal muscle humans. To determine whether pre‐exercise glycogen content influences the magnitude and/or duration this adaptive response, six male subjects performed one‐legged cycling to lower one leg and then, following day, completed 2.5 h low intensity two‐legged exercise. Nuclei mRNA were isolated biopsies obtained vastus lateralis control reduced (pre‐exercise = 609 ± 47 337 33 mmol kg −1 dry weight, respectively) legs before after 0, 2 5 recovery. Exercise a significant ( P < 0.05 ) increase (2‐ 3‐fold) transcription pyruvate dehydrogenase kinase 4 (PDK4) uncoupling protein 3 (UCP3) only. Although PDK4, lipoprotein lipase (LPL) hexokinase II (HKII) elevated exercise, no consistent difference was found between two response In second study, trials (separated by weeks) consisting knee extensor with either (398 52 weight) or (240 38 glycogen. significantly greater PDK4 (> 6‐fold) than (< trial. Induction UCP3 also signficantly higher (11.4‐ 3.5‐fold, (5.0‐ 1.7‐fold, These data indicate that enhances transcriptional activation some raising possibility signalling mechanisms sensitive FFA availability may be linked exercise‐responsive genes.