Sex differences in the alterations of Na+,K+‐ATPase following ischaemia–reperfusion injury in the rat kidney

• DOI: 10.1113/jphysiol.2003.054825
• Publication Date: 2003-12-16T01:23:40Z
• Authors: Andrea Fekete; Ádám Vannay; Ágota Vér; Barna Vásárhelyi; Veronika Müller; Nengtai Ouyang; György Reusz; Tivadar Tulassay; Attila J. Szabó
• Keywords: Blood urea nitrogen; Pathogenesis; Histology

Postischaemic acute renal failure (ARF) is influenced by sex. Na(+), K(+)-ATPase (NKA) plays a crucial role in the pathogenesis of postischaemic ARF. We tested impact sex on mRNA, protein expression, cellular distribution and enzyme activity NKA following ischaemia-reperfusion (I-R) injury. The left pedicle uninephrectomized female (F) male (M) Wistar rats was clamped for 55 min followed 2 h (T2) 16 (T16) reperfusion. Uninephrectomized, sham-operated F M served as controls (n= 6 per group). Blood urea nitrogen, serum creatinine histology were evaluated to detect severity mRNA expression alpha1 beta1 subunits detected RT-PCR. effect I-R compared Triton X-100 extraction. Cellular proteins divided into Triton-insoluble Triton-soluble fractions assessed Western blot. also determined. After ischaemic insult blood nitrogen higher showed more rapid progression versus (P < 0.05). subunit decreased groups controls, but both control However, levels total tissue homogenate did not differ extractability lower at T16 same (T2: 14.9 +/- 2.3 9.15 2.21 U) (T16: 11.7 4.1 5.65 U; P between any protocol. concluded that protected from detrimental effects injury females. Higher might be additional contributing factors improved function rats.