Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during lipopolysaccharide‐induced acute inflammation

• DOI: 10.1113/jphysiol.2006.104711
• Publication Date: 2006-03-10T01:32:57Z
• Authors: Athanasia Bletsa; Ellen Berggreen; Inge Fristad; Olav Tenstad; Helge Wiig
• Keywords: Interstitial fluid; Tissue fluid

The dental pulp consists of loose connective tissue encased in rigid dentinal walls. Because its topography the has low interstitial compliance and limited capacity to expand during fluid volume changes. Due limitations regarding access fluid, basic knowledge on transcapillary transport parameters is lacking for this organ. scope project was dual: first we aimed at establishing a method isolation (IF), second applied rats subjected lipopolysaccharide (LPS)-induced endotoxaemia. aim measure colloid osmotic pressure (COP) pro-inflammatory cytokines IF acute inflammation. Fluid volumes pulpal blood flow (PBF) were measured obtain more information about microcirculatory changes that take place pulpitis model. By centrifugation incisor 239 g able extract representative IF. Pulp had relative high control COP (approximately 83% plasma COP) similar 3 h after LPS challenge. exhibited content (0.60 +/- 0.03 ml (g wet weight)(-1)) vascular 0.01 w.w.)(-1) No differences observed distribution 1.5 exposure. PBF systemic dropped significantly administration. remained whereas re-established 3-h period, implying organ dysfunction. There differential pattern cytokine expression serum with such as IL-1alpha, IL-1beta TNF-alpha locally produced, others IFN-gamma IL-6 produced systemically probably spilled over Our findings show can be isolated by useful when studying balance extracellular signalling mechanisms normal pathological conditions.